| Position | Starters | Team | Games | Opponents |
| C | Edgar Quero | ChA | 6 | Mil(3) Hou(3) |
| 1B | Pete Alonso | NYN | 8 | @Was(1) Ari(3) @StL(4) |
| 2B (3B) | Jordan Westburg | Bal | 6 | NYA(3) KC(3) |
| SS | Bobby Witt Jr. | KC | 6 | @TB(3) @Bal(3) |
| 3B | Noelvi Marte | Cin | 8 | StL(5) Was(3) |
| OF | Austin Hays | Cin | 8 | StL(5) Was(3) |
| OF | Fernando Tatis Jr. | SD | 5 | SF(2) @Pit(3) |
| OF | Pete Crow-Armstrong | ChN | 6 | @Pit(3) @Mil(3) |
| DH | Shohei Ohtani | LAD | 6 | Mia(3) @Atl(3) |
| Position | 1st Backup | Team | Games | Opponents |
| 1B | Kyle Manzardo | Cle | 7 | Min(4) @Tor(3) |
| 2B (SS) | Jackson Holliday | Bal | 6 | NYA(3) KC(3) |
| SS | Jacob Wilson | Oak | 7 | @Tex(4) @Mia(3) |
| 3B | Rafael Devers | Bos | 6 | @Tor(3) Min(3) |
| OF | Dylan Crews | Was | 7 | NYN(1) @Phi(3) @Cin(3) |
| DH (OF) | Jordan Beck | Col | 7 | Atl(3) @SF(4) |
| Position | 2nd Backup | Team | Games | Opponents |
| 1B | Nick Kurtz | Oak | 7 | @Tex(4) @Mia(3) |
| DH (3B) | Caleb Durbin | Mil | 6 | @ChA(3) ChN(3) |
| Position | 3rd Backup | Team | Games | Opponents |
| SS | Sebastian Walcott | Tex | 7 | Oak(4) Sea(3) |
| Position | Bench | Team | Games | Opponents |
| 2B | Travis Bazzana | Cle | 7 | Min(4) @Tor(3) |
| SS | Marcelo Mayer | Bos | 6 | @Tor(3) Min(3) |
| Pitching Staff Alignment |
| 5 Starts, 3 Relievers |
| Position | Pitching Order | Team | Games | Opponents | Next Scheduled Start |
| 1st SP | Tyler Glasnow | LAD | 6 | Mia(3) @Atl(3) | Last Pitched Sunday April 27th |
| 2nd SP | Roki Sasaki | LAD | 6 | Mia(3) @Atl(3) | Last Pitched Saturday May 3rd |
| 3rd SP | Jackson Jobe | Det | 7 | @Hou(3) @LAA(4) | Last Pitched Wednesday April 30th |
| 4th SP | Clay Holmes | NYN | 8 | @Was(1) Ari(3) @StL(4) | Last Pitched Friday May 2nd |
| 5th SP | Zebby Matthews | Min | 7 | @Cle(4) @Bos(3) | Has Not Played Yet |
| 6th SP | Clarke Schmidt | NYA | 6 | @Bal(3) TB(3) | Last Pitched Sunday April 27th |
| 7th SP | Luke Weaver | NYA | 6 | @Bal(3) TB(3) | Last Pitched Friday May 2nd |
| 1st RP | Emmanuel Clase | Cle | 7 | Min(4) @Tor(3) | Last Pitched Saturday May 3rd |
| 2nd RP | Edwin Diaz | NYN | 8 | @Was(1) Ari(3) @StL(4) | Last Pitched Saturday April 26th |
| 3rd RP | Tanner Scott | LAD | 6 | Mia(3) @Atl(3) | Last Pitched Friday May 2nd |
| 4th RP | Luke Jackson | Tex | 7 | Oak(4) Sea(3) | Last Pitched Wednesday April 30th |
| Position | Injured List | Team | Games | Opponents |
| SP | Jack Leiter | Tex | 7 | Oak(4) Sea(3) |