| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Sean Murphy | C | Atl | 30 | |
| Pete Alonso | 1B | NYN | 30 | |
| Jonathan Aranda | 1B | TB | 26 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Ben Rice | 1B | NYA | 26 | |
| Christian Moore | 2B | LAA | 22 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Jordan Lawlar | SS | Ari | 22 | |
| Rafael Devers | 3B | Bos | 28 | |
| Ryan McMahon | 3B | Col | 30 | |
| Chase Meidroth | 3B (2B,SS) | ChA | N/A | |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Yordan Alvarez | OF | Hou | 27 | |
| Lawrence Butler | OF | Oak | 24 | |
| Kerry Carpenter | OF | Det | 27 | |
| Oneil Cruz | OF | Pit | 26 | |
| Hunter Goodman | OF (C) | Col | 25 | |
| Wyatt Langford | OF | Tex | 23 | |
| Kyle Schwarber | OF | Phi | 32 | |
| Spencer Arrighetti | SP | Hou | N/A | April 08, 2025:15 Day IL |
| Kris Bubic | SP | KC | 27 | |
| Tony Gonsolin | SP | LAD | 30 | |
| MacKenzie Gore | SP | Was | 26 | |
| Zebby Matthews | SP | Min | N/A | |
| Dustin May | SP | LAD | 27 | |
| Tylor Megill | SP | NYN | 29 | |
| Reese Olson | SP | Det | 25 | |
| Cole Ragans | SP | KC | 27 | |
| Drew Rasmussen | SP | TB | 29 | |
| Joe Ryan | SP | Min | 28 | |
| Ryan Weathers | SP | Mia | 25 | March 27, 2025:15 Day IL |
| Jhoan Duran | RP | Min | 27 | |
| Jeff Hoffman | RP | Tor | 32 | |
| Andres Munoz | RP | Sea | 26 |