| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Salvador Perez | C (C,1B) | KC | 34 | |
| Keibert Ruiz | C | Was | 26 | |
| Jac Caglianone | 1B | KC | 22 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Matt Mervis | 1B | Mia | 27 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Travis Bazzana | 2B | Cle | 22 | |
| Jake Cronenworth | 2B | SD | 31 | April 11, 2025:10 Day IL |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Brice Turang | 2B | Mil | 25 | |
| Ezequiel Tovar | SS | Col | 23 | April 19, 2025:10 Day IL |
| Jacob Wilson | SS | Oak | 23 | |
| Josh Jung | 3B | Tex | 27 | |
| Matt Shaw | 3B | ChN | 23 | |
| Mark Vientos | 3B | NYN | 25 | |
| Roman Anthony | OF | Bos | 20 | |
| TJ Friedl | OF | Cin | 29 | |
| Michael Harris II | OF | Atl | 24 | |
| Justyn-Henry Malloy | OF | Det | 25 | |
| Jackson Merrill | OF | SD | 22 | April 08, 2025:10 Day IL |
| Andy Pages | OF | LAD | 24 | |
| Heliot Ramos | OF | SF | 25 | |
| Tim Tawa | OF (2B) | Ari | N/A | |
| Andrew Heaney | SP | Pit | 33 | |
| Matthew Liberatore | SP | StL | 25 | |
| Nick Lodolo | SP | Cin | 27 | |
| Pablo Lopez | SP | Min | 29 | |
| Tylor Megill | SP | NYN | 29 | |
| Landen Roupp | SP | SF | N/A | |
| Spencer Schwellenbach | SP | Atl | 24 | |
| José Soriano | SP | LAA | 26 | |
| Spencer Strider | SP | Atl | 26 | April 21, 2025:15 Day IL |
| Ryan Helsley | RP | StL | 30 | |
| Mason Miller | RP | Oak | 26 |