| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Francisco Álvarez | C | NYN | 23 | |
| Samuel Basallo | C | Bal | 21 | |
| Kyle Teel | C | ChA | 23 | |
| Jac Caglianone | 1B (OF) | KC | 22 | |
| Bryce Eldridge | 1B | SF | 21 | |
| Nick Kurtz | 1B | Oak | 22 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Travis Bazzana | 2B | Cle | 23 | |
| Luke Keaschall | 2B | Min | 23 | September 25, 2025:10 Day IL |
| Brice Turang | 2B | Mil | 25 | |
| Marcelo Mayer | SS (2B,3B) | Bos | 22 | July 25, 2025:60 Day IL |
| Ezequiel Tovar | SS | Col | 24 | |
| Jacob Wilson | SS | Oak | 23 | |
| Josh Jung | 3B | Tex | 27 | |
| Matt Shaw | 3B | ChN | 23 | |
| Cam Smith | 3B (OF) | Hou | 22 | |
| TJ Friedl | OF | Cin | 30 | |
| Michael Harris II | OF | Atl | 24 | |
| Jackson Merrill | OF | SD | 22 | |
| Andy Pages | OF | LAD | 24 | |
| Jurickson Profar | OF | Atl | 32 | |
| Heliot Ramos | OF | SF | 26 | |
| Chase Burns | SP (RP) | Cin | 22 | |
| Luis Gil | SP | NYA | 27 | |
| MacKenzie Gore | SP | Was | 26 | September 23, 2025:15 Day IL |
| Jack Leiter | SP | Tex | N/A | |
| Jacob Misiorowski | SP | Mil | N/A | |
| Andrew Painter | SP | Phi | N/A | |
| Landen Roupp | SP | SF | N/A | August 22, 2025:15 Day IL |
| José Soriano | SP | LAA | 27 | September 18, 2025:15 Day IL |
| Spencer Strider | SP | Atl | 26 | |
| Ryan Helsley | RP | NYN | 31 | |
| Mason Miller | RP | SD | 27 |