| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| William Contreras | C | Mil | 27 | |
| Carson Kelly | C | ChN | 30 | |
| Willson Contreras | 1B | StL | 32 | |
| Deyvison De Los Santos | 1B | Mia | 21 | |
| Paul Goldschmidt | 1B | NYA | 37 | |
| Nolan Schanuel | 1B | LAA | 23 | |
| Jose Altuve | 2B (OF) | Hou | 34 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Zach McKinstry | 2B (3B,OF) | Det | 30 | |
| Willy Adames | SS | SF | 29 | |
| Adael Amador | SS (2B) | Col | N/A | |
| Anthony Volpe | SS | NYA | 24 | |
| Alex Freeland | 3B | LAD | N/A | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Chase Meidroth | 3B (2B,SS) | ChA | N/A | |
| Joey Ortiz | 3B (SS) | Mil | 26 | |
| Yordan Alvarez | OF | Hou | 27 | |
| Trent Grisham | OF | NYA | 28 | |
| Chandler Simpson | OF | TB | 24 | |
| George Springer | OF | Tor | 35 | |
| Fernando Tatis Jr. | OF | SD | 26 | |
| Alex Verdugo | OF | Atl | 28 | |
| Chase Dollander | SP | Col | N/A | |
| Max Fried | SP | NYA | 31 | |
| Luis Gil | SP | NYA | 26 | March 24, 2025:60 Day IL |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Michael King | SP | SD | 29 | |
| Jack Leiter | SP | Tex | N/A | |
| Reese Olson | SP | Det | 25 | |
| David Peterson | SP | NYN | 29 | |
| Zack Wheeler | SP | Phi | 34 | |
| Camilo Doval | RP | SF | 27 | |
| Pete Fairbanks | RP | TB | 31 | |
| Kyle Finnegan | RP | Was | 33 |