| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Jonah Heim | C | Tex | 29 | |
| Deyvison De Los Santos | 1B | Mia | 21 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Nick Kurtz | 1B | Oak | 22 | |
| Michael Toglia | 1B | Col | 26 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Ketel Marte | 2B | Ari | 31 | |
| Kyren Paris | SS (2B,OF) | LAA | 23 | |
| Trevor Story | SS | Bos | 32 | |
| Ezequiel Tovar | SS | Col | 23 | April 19, 2025:10 Day IL |
| Josh Jung | 3B | Tex | 27 | |
| Noelvi Marte | 3B | Cin | 23 | |
| Max Muncy | 3B | LAD | 34 | |
| Connor Norby | 3B | Mia | 24 | |
| Byron Buxton | OF | Min | 31 | |
| Michael Harris II | OF | Atl | 24 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Chandler Simpson | OF | TB | 24 | |
| Pavin Smith | OF (1B) | Ari | 29 | |
| Tim Tawa | OF (2B) | Ari | N/A | |
| Colby Thomas | OF | Oak | 24 | |
| Christian Yelich | OF | Mil | 33 | |
| Shane Baz | SP | TB | 25 | |
| Taj Bradley | SP | TB | 24 | |
| Max Fried | SP | NYA | 31 | |
| Ryan Gusto | SP (RP) | Hou | N/A | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Matthew Liberatore | SP | StL | 25 | |
| Jacob Misiorowski | SP | Mil | N/A | |
| Casey Mize | SP | Det | 28 | |
| Carlos Estevez | RP | KC | 32 | |
| Luke Jackson | RP | Tex | 33 | |
| Tanner Scott | RP | LAD | 30 |