| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Samuel Basallo | C | Bal | 20 | |
| Ryan Jeffers | C | Min | 27 | |
| Alejandro Kirk | C | Tor | 26 | |
| Pete Alonso | 1B | NYN | 30 | |
| Matt Mervis | 1B | Mia | 27 | |
| Ozzie Albies | 2B | Atl | 28 | |
| Wilmer Flores | 2B | SF | 33 | |
| Nick Gonzales | 2B | Pit | 25 | March 28, 2025:10 Day IL |
| Adael Amador | SS (2B) | Col | N/A | |
| Francisco Lindor | SS | NYN | 31 | |
| Alec Bohm | 3B | Phi | 28 | |
| Joey Ortiz | 3B (SS) | Mil | 26 | |
| Roman Anthony | OF | Bos | 20 | |
| Riley Greene | OF | Det | 24 | |
| Trent Grisham | OF | NYA | 28 | |
| Heston Kjerstad | OF | Bal | 26 | |
| Heliot Ramos | OF | SF | 25 | |
| Victor Scott | OF | StL | 24 | |
| Alek Thomas | OF | Ari | 25 | |
| Mike Trout | OF | LAA | 33 | |
| Alex Verdugo | OF | Atl | 28 | |
| Pat Corbin | SP | Tex | 35 | |
| Yu Darvish | SP | SD | 38 | March 27, 2025:15 Day IL |
| Chase Dollander | SP | Col | N/A | |
| Erick Fedde | SP | StL | 32 | |
| Kevin Gausman | SP | Tor | 34 | |
| Hunter Greene | SP | Cin | 25 | |
| Tylor Megill | SP | NYN | 29 | |
| Aaron Nola | SP | Phi | 31 | |
| Luis Ortiz | SP | Cle | 26 | |
| Tarik Skubal | SP | Det | 28 | |
| Kenley Jansen | RP | LAA | 37 | |
| Justin Martinez | RP | Ari | 23 | May 01, 2025:15 Day IL |