| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Pete Alonso | 1B | NYN | 30 | |
| Jonathan Aranda | 1B | TB | 26 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Otto Lopez | 2B | Mia | 26 | |
| Brandon Lowe | 2B | TB | 30 | |
| Christian Moore | 2B | LAA | 22 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Dansby Swanson | SS | ChN | 31 | |
| Coby Mayo | 3B | Bal | 23 | |
| Ryan McMahon | 3B | Col | 30 | |
| Jordan Westburg | 3B (2B) | Bal | 26 | April 28, 2025:10 Day IL |
| Lawrence Butler | OF | Oak | 24 | |
| Kerry Carpenter | OF | Det | 27 | |
| Oneil Cruz | OF | Pit | 26 | |
| Hunter Goodman | OF (C) | Col | 25 | |
| Julio Rodriguez | OF | Sea | 24 | |
| James Wood | OF | Was | 22 | |
| Kris Bubic | SP | KC | 27 | |
| MacKenzie Gore | SP | Was | 26 | |
| Michael King | SP | SD | 29 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Nick Lodolo | SP | Cin | 27 | |
| Reese Olson | SP | Det | 25 | |
| Brandon Pfaadt | SP | Ari | 26 | |
| Nick Pivetta | SP | SD | 32 | |
| Joe Ryan | SP | Min | 28 | |
| Tarik Skubal | SP | Det | 28 | |
| Shane Smith | SP | ChA | N/A | |
| Gavin Williams | SP | Cle | 25 | |
| Brandon Woodruff | SP | Mil | 32 | March 27, 2025:15 Day IL |
| Jeff Hoffman | RP | Tor | 32 | |
| Emilio Pagan | RP | Cin | 33 | |
| Ryan Walker | RP | SF | N/A |