| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Joey Bart | C | Pit | 28 | |
| Ryan Jeffers | C | Min | 27 | |
| Jake Burger | 1B | Tex | 29 | |
| Vinnie Pasquantino | 1B | KC | 27 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Otto Lopez | 2B | Mia | 26 | |
| Marcus Semien | 2B | Tex | 34 | |
| Gleyber Torres | 2B | Det | 28 | |
| J.P. Crawford | SS | Sea | 30 | |
| Xavier Edwards | SS | Mia | 25 | |
| Ezequiel Tovar | SS | Col | 23 | April 19, 2025:10 Day IL |
| Isaac Paredes | 3B | Hou | 26 | |
| Matt Shaw | 3B | ChN | 23 | |
| Mark Vientos | 3B | NYN | 25 | |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Lourdes Gurriel | OF | Ari | 31 | |
| Wyatt Langford | OF | Tex | 23 | |
| Jackson Merrill | OF | SD | 22 | April 08, 2025:10 Day IL |
| Seiya Suzuki | OF | ChN | 30 | |
| Chase Burns | SP | Cin | 22 | |
| Bubba Chandler | SP | Pit | N/A | |
| Jack Flaherty | SP | Det | 29 | |
| Gunnar Hoglund | SP | Oak | 25 | |
| Michael King | SP | SD | 29 | |
| Bailey Ober | SP | Min | 29 | |
| Nick Pivetta | SP | SD | 32 | |
| Jose Quintana | SP | Mil | 36 | |
| Robbie Ray | SP | SF | 33 | |
| Roki Sasaki | SP | LAD | 23 | |
| Spencer Strider | SP | Atl | 26 | April 21, 2025:15 Day IL |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Pete Fairbanks | RP | TB | 31 | |
| Luke Jackson | RP | Tex | 33 |