| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Adley Rutschman | C | Bal | 27 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Kyle Manzardo | 1B | Cle | 24 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Christian Moore | 2B | LAA | 22 | |
| Bryson Stott | 2B | Phi | 27 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Jeremy Pena | SS | Hou | 27 | |
| Masyn Winn | SS | StL | 23 | |
| Alex Freeland | 3B | LAD | N/A | |
| Noelvi Marte | 3B | Cin | 23 | |
| Matt Shaw | 3B | ChN | 23 | |
| Mark Vientos | 3B | NYN | 25 | |
| Roman Anthony | OF | Bos | 20 | |
| Byron Buxton | OF | Min | 31 | |
| Evan Carter | OF | Tex | 22 | |
| Colton Cowser | OF | Bal | 25 | March 31, 2025:60 Day IL-Left thumb fracture |
| Sal Frelick | OF | Mil | 25 | |
| Chandler Simpson | OF | TB | 24 | |
| Alex Verdugo | OF | Atl | 28 | |
| Jordan Walker | OF | StL | 22 | |
| Gerrit Cole | SP | NYA | 34 | March 22, 2025:60 Day IL |
| Grant Holmes | SP | Atl | N/A | |
| Jack Kochanowicz | SP | LAA | N/A | |
| Matthew Liberatore | SP | StL | 25 | |
| Shane McClanahan | SP | TB | 28 | March 26, 2025:60 Day IL-Ab strain |
| Mitchell Parker | SP | Was | 25 | |
| Luis Severino | SP | Oak | 31 | |
| José Soriano | SP | LAA | 26 | |
| Michael Wacha | SP | KC | 33 | |
| Justin Martinez | RP | Ari | 23 | May 01, 2025:15 Day IL |
| Ryan Walker | RP | SF | N/A |