| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Jonah Heim | C | Tex | 29 | |
| Ivan Herrera | C | StL | 24 | April 07, 2025:15 Day IL-Knee |
| Triston Casas | 1B | Bos | 25 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Ben Rice | 1B | NYA | 26 | |
| Travis Bazzana | 2B | Cle | 22 | |
| Christian Moore | 2B | LAA | 22 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Sebastian Walcott | SS | Tex | 19 | |
| Brooks Baldwin | 3B (2B,SS,OF) | ChA | N/A | |
| Caleb Durbin | 3B | Mil | 25 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Noelvi Marte | 3B | Cin | 23 | |
| Coby Mayo | 3B | Bal | 23 | |
| Wilyer Abreu | OF | Bos | 25 | |
| Roman Anthony | OF | Bos | 20 | |
| Evan Carter | OF | Tex | 22 | |
| Michael Harris II | OF | Atl | 24 | |
| Anthony Santander | OF | Tor | 30 | |
| Chandler Simpson | OF | TB | 24 | |
| Pavin Smith | OF (1B) | Ari | 29 | |
| Zac Veen | OF | Col | N/A | |
| Chase Dollander | SP | Col | N/A | |
| Erick Fedde | SP | StL | 32 | |
| Ryan Gusto | SP (RP) | Hou | N/A | |
| Tanner Houck | SP | Bos | 28 | |
| Matthew Liberatore | SP | StL | 25 | |
| Andrew Painter | SP | Phi | N/A | |
| Eury Perez | SP | Mia | 22 | February 20, 2025:60 Day IL |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Hayden Wesneski | SP | Hou | 27 | |
| Jhoan Duran | RP | Min | 27 | |
| Raisel Iglesias | RP | Atl | 35 |