| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Samuel Basallo | C | Bal | 20 | |
| Agustin Ramirez | C | Mia | 23 | |
| Jake Burger | 1B | Tex | 29 | |
| Triston Casas | 1B | Bos | 25 | |
| Nolan Schanuel | 1B | LAA | 23 | |
| Matt McLain | 2B | Cin | 25 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Kyren Paris | SS (2B,OF) | LAA | 23 | |
| Anthony Volpe | SS | NYA | 24 | |
| Alex Bregman | 3B | Bos | 31 | |
| Caleb Durbin | 3B | Mil | 25 | |
| Brady House | 3B | Was | 21 | |
| Ryan McMahon | 3B | Col | 30 | |
| Evan Carter | OF | Tex | 22 | |
| Jasson Dominguez | OF | NYA | 22 | |
| Trent Grisham | OF | NYA | 28 | |
| Heston Kjerstad | OF | Bal | 26 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Chandler Simpson | OF | TB | 24 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Daulton Varsho | OF | Tor | 28 | |
| Alex Verdugo | OF | Atl | 28 | |
| Taylor Ward | OF | LAA | 31 | |
| Andrew Abbott | SP | Cin | 25 | |
| Taj Bradley | SP | TB | 24 | |
| Dylan Cease | SP | SD | 29 | |
| Andrew Heaney | SP | Pit | 33 | |
| Matthew Liberatore | SP | StL | 25 | |
| Max Meyer | SP | Mia | 26 | |
| Roki Sasaki | SP | LAD | 23 | |
| Felix Bautista | RP | Bal | 29 | |
| Edwin Diaz | RP | NYN | 31 | |
| Ryan Helsley | RP | StL | 30 |