| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Yainer Diaz | C | Hou | 26 | |
| Ryan Jeffers | C | Min | 27 | |
| Michael Busch | 1B | ChN | 27 | |
| Colt Keith | 1B (2B) | Det | 23 | |
| Brendan Donovan | 2B (SS,OF) | StL | 28 | |
| Luis Garcia | 2B | Was | 24 | |
| Brooks Lee | 2B (3B) | Min | 24 | |
| Gavin Lux | 2B (OF) | Cin | 27 | |
| Javier Baez | SS (3B,OF) | Det | 32 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Ha-seong Kim | SS | TB | 29 | March 27, 2025:10 Day IL |
| Max Muncy | SS (2B) | Oak | 22 | |
| Carson Williams | SS | TB | 21 | |
| Rafael Devers | 3B | Bos | 28 | |
| Matt Shaw | 3B | ChN | 23 | |
| Nick Castellanos | OF | Phi | 33 | |
| Pete Crow-Armstrong | OF | ChN | 23 | |
| Aaron Judge | OF | NYA | 33 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Kyle Stowers | OF | Mia | 27 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Brayan Bello | SP | Bos | 25 | |
| Taj Bradley | SP | TB | 24 | |
| Griffin Canning | SP | NYN | 28 | |
| Jacob deGrom | SP | Tex | 36 | |
| Logan Gilbert | SP | Sea | 27 | April 26, 2025:15 Day IL |
| Mike Lorenzen | SP | KC | 33 | |
| Shane McClanahan | SP | TB | 28 | March 26, 2025:60 Day IL-Ab strain |
| Mitchell Parker | SP | Was | 25 | |
| Kumar Rocker | SP | Tex | N/A | April 25, 2025:15 Day IL |
| Justin Steele | SP | ChN | 29 | April 09, 2025:60 Day IL |
| Logan Webb | SP | SF | 28 | |
| Pete Fairbanks | RP | TB | 31 | |
| Josh Hader | RP | Hou | 31 |