| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Ryan Jeffers | C | Min | 27 | |
| Keibert Ruiz | C | Was | 26 | |
| Willson Contreras | 1B | StL | 32 | |
| Nathaniel Lowe | 1B | Was | 29 | |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Brandon Lowe | 2B | TB | 30 | |
| Thomas Saggese | 2B (SS) | StL | 23 | |
| J.P. Crawford | SS | Sea | 30 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Amed Rosario | SS (2B,3B) | Was | 29 | |
| Trea Turner | SS | Phi | 31 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Isaac Paredes | 3B | Hou | 26 | |
| Jorge Polanco | 3B | Sea | 31 | |
| Lourdes Gurriel | OF | Ari | 31 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Trevor Larnach | OF | Min | 28 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Kyle Schwarber | OF | Phi | 32 | |
| Victor Scott | OF | StL | 24 | |
| Kyle Tucker | OF | ChN | 28 | |
| Kris Bubic | SP | KC | 27 | |
| Bubba Chandler | SP | Pit | N/A | |
| Max Fried | SP | NYA | 31 | |
| Logan Henderson | SP | Mil | N/A | |
| Luis Ortiz | SP | Cle | 26 | |
| Eury Perez | SP | Mia | 22 | February 20, 2025:60 Day IL |
| Cristopher Sanchez | SP | Phi | 28 | |
| Spencer Schwellenbach | SP | Atl | 24 | |
| Jameson Taillon | SP | ChN | 33 | |
| Hayden Wesneski | SP | Hou | 27 | |
| Camilo Doval | RP | SF | 27 | |
| Raisel Iglesias | RP | Atl | 35 | |
| Ryan Walker | RP | SF | N/A |