| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Yainer Diaz | C | Hou | 26 | |
| Gabriel Moreno | C | Ari | 25 | |
| Ty France | 1B | Min | 30 | |
| Ryan Mountcastle | 1B | Bal | 28 | |
| Michael Toglia | 1B | Col | 26 | |
| Otto Lopez | 2B | Mia | 26 | |
| Luis Rengifo | 2B (3B) | LAA | 28 | |
| Xavier Edwards | SS | Mia | 25 | |
| Corey Seager | SS | Tex | 31 | April 23, 2025:10 Day IL |
| Alec Bohm | 3B | Phi | 28 | |
| Joey Ortiz | 3B (SS) | Mil | 26 | |
| Ben Williamson | 3B | Sea | N/A | |
| Byron Buxton | OF | Min | 31 | |
| Max Kepler | OF | Phi | 32 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Christopher Morel | OF | TB | 25 | |
| Brandon Nimmo | OF | NYN | 32 | |
| Ceddanne Rafaela | OF | Bos | 24 | |
| Bryan Reynolds | OF | Pit | 30 | |
| Spencer Steer | OF (1B) | Cin | 27 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Mike Yastrzemski | OF | SF | 34 | |
| Taj Bradley | SP | TB | 24 | |
| Hunter Brown | SP | Hou | 26 | |
| Bowden Francis | SP | Tor | 29 | |
| Zac Gallen | SP | Ari | 29 | |
| Aaron Nola | SP | Phi | 31 | |
| Andrew Painter | SP | Phi | N/A | |
| Robbie Ray | SP | SF | 33 | |
| JP Sears | SP | Oak | 29 | |
| Brady Singer | SP | Cin | 28 | |
| Ranger Suarez | SP | Phi | 29 | March 23, 2025:15 Day IL |
| Jhoan Duran | RP | Min | 27 | |
| Josh Hader | RP | Hou | 31 |