| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Gabriel Moreno | C | Ari | 25 | |
| Adley Rutschman | C | Bal | 27 | |
| Vladimir Guerrero Jr. | 1B | Tor | 26 | |
| Nathaniel Lowe | 1B | Was | 29 | |
| Andrés Gimenez | 2B | Tor | 26 | |
| Ketel Marte | 2B | Ari | 31 | |
| Gabriel Arias | SS (2B,3B) | Cle | 25 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Dansby Swanson | SS | ChN | 31 | |
| Matt Chapman | 3B | SF | 32 | |
| Ryan McMahon | 3B | Col | 30 | |
| Lawrence Butler | OF | Oak | 24 | |
| Oneil Cruz | OF | Pit | 26 | |
| Sal Frelick | OF | Mil | 25 | |
| Riley Greene | OF | Det | 24 | |
| Trent Grisham | OF | NYA | 28 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Juan Soto | OF | NYN | 26 | |
| Griffin Canning | SP | NYN | 28 | |
| Jack Flaherty | SP | Det | 29 | |
| MacKenzie Gore | SP | Was | 26 | |
| Hunter Greene | SP | Cin | 25 | |
| Tanner Houck | SP | Bos | 28 | |
| Yusei Kikuchi | SP | LAA | 33 | |
| Reese Olson | SP | Det | 25 | |
| Ryan Pepiot | SP | TB | 27 | |
| Eury Perez | SP | Mia | 22 | February 20, 2025:60 Day IL |
| Carlos Rodon | SP | NYA | 32 | |
| Eduardo Rodriguez | SP | Ari | 32 | |
| Randy Vasquez | SP | SD | 26 | |
| Bryan Woo | SP | Sea | 25 | |
| Brandon Woodruff | SP | Mil | 32 | March 27, 2025:15 Day IL |
| Ryan Pressly | RP | ChN | 36 | |
| Abner Uribe | RP | Mil | 24 |