| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Jonah Heim | C | Tex | 29 | |
| Alejandro Kirk | C | Tor | 26 | |
| Tyler Stephenson | C | Cin | 28 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Rhys Hoskins | 1B | Mil | 32 | |
| Matt Olson | 1B | Atl | 31 | |
| Ozzie Albies | 2B | Atl | 28 | |
| Kristian Campbell | 2B (OF) | Bos | 22 | |
| Thairo Estrada | 2B | Col | 29 | March 27, 2025:60 Day IL |
| Bo Bichette | SS | Tor | 27 | |
| Jose Caballero | SS (3B,OF) | TB | 28 | |
| Carlos Correa | SS | Min | 30 | |
| Max Muncy | 3B | LAD | 34 | |
| Matt Vierling | 3B | Det | 28 | March 27, 2025:10 Day IL |
| Cody Bellinger | OF | NYA | 29 | |
| Alec Burleson | OF (1B) | StL | 26 | |
| Byron Buxton | OF | Min | 31 | |
| Evan Carter | OF | Tex | 22 | |
| Nick Castellanos | OF | Phi | 33 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Marcell Ozuna | DH | Atl | 34 | |
| Spencer Arrighetti | SP | Hou | N/A | April 08, 2025:15 Day IL |
| Shane Baz | SP | TB | 25 | |
| Max Fried | SP | NYA | 31 | |
| Zac Gallen | SP | Ari | 29 | |
| Kevin Gausman | SP | Tor | 34 | |
| Jared Jones | SP | Pit | 23 | March 27, 2025:60 Day IL |
| Mitch Keller | SP | Pit | 29 | |
| Brandon Pfaadt | SP | Ari | 26 | |
| Paul Skenes | SP | Pit | 22 | |
| Framber Valdez | SP | Hou | 31 | |
| Kenley Jansen | RP | LAA | 37 | |
| Robert Suarez | RP | SD | 34 | |
| Ryan Walker | RP | SF | N/A |