| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Edgar Quero | C | ChA | 22 | |
| Pete Alonso | 1B | NYN | 30 | |
| Nick Kurtz | 1B | Oak | 22 | |
| Kyle Manzardo | 1B | Cle | 24 | |
| Travis Bazzana | 2B | Cle | 22 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Jeff McNeil | 2B | NYN | 33 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Sebastian Walcott | SS | Tex | 19 | |
| Jacob Wilson | SS | Oak | 23 | |
| Bobby Witt Jr. | SS | KC | 24 | |
| Rafael Devers | 3B | Bos | 28 | |
| Caleb Durbin | 3B | Mil | 25 | |
| Noelvi Marte | 3B | Cin | 23 | |
| Jordan Westburg | 3B (2B) | Bal | 26 | April 28, 2025:10 Day IL |
| Jordan Beck | OF | Col | 24 | |
| Dylan Crews | OF | Was | 23 | |
| Pete Crow-Armstrong | OF | ChN | 23 | |
| Austin Hays | OF | Cin | 29 | May 02, 2025:10 Day IL |
| Fernando Tatis Jr. | OF | SD | 26 | |
| Shohei Ohtani | DH ,SP | LAD | 30 | |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Tony Gonsolin | SP | LAD | 30 | |
| Clay Holmes | SP | NYN | 32 | |
| Jackson Jobe | SP | Det | 22 | |
| Jack Leiter | SP | Tex | N/A | |
| Zebby Matthews | SP | Min | N/A | |
| Roki Sasaki | SP | LAD | 23 | |
| Clarke Schmidt | SP | NYA | 29 | |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Emmanuel Clase | RP | Cle | 27 | |
| Edwin Diaz | RP | NYN | 31 | |
| Luke Jackson | RP | Tex | 33 | |
| Tanner Scott | RP | LAD | 30 |