| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Joey Bart | C | Pit | 28 | |
| Cal Raleigh | C | Sea | 28 | |
| Jonathan Aranda | 1B | TB | 26 | |
| Spencer Torkelson | 1B | Det | 25 | |
| Wilmer Flores | 2B | SF | 33 | |
| Romy Gonzalez | 2B (1B) | Bos | 28 | |
| Bo Bichette | SS | Tor | 27 | |
| Xavier Edwards | SS | Mia | 25 | |
| Masyn Winn | SS | StL | 23 | |
| Nolan Arenado | 3B | StL | 34 | |
| Dylan Moore | 3B (2B,OF) | Sea | 32 | April 29, 2025:10 Day IL |
| Isaac Paredes | 3B | Hou | 26 | |
| Adolis Garcia | OF | Tex | 32 | |
| Hunter Goodman | OF (C) | Col | 25 | |
| Austin Hays | OF | Cin | 29 | May 02, 2025:10 Day IL |
| Andy Pages | OF | LAD | 24 | |
| Heliot Ramos | OF | SF | 25 | |
| Kyle Stowers | OF | Mia | 27 | |
| Matt Wallner | OF | Min | 27 | April 17, 2025:10 Day IL |
| Marcell Ozuna | DH | Atl | 34 | |
| Chris Bassitt | SP | Tor | 36 | |
| Jose Berrios | SP | Tor | 30 | |
| Noah Cameron | SP | KC | N/A | |
| Hunter Greene | SP | Cin | 25 | |
| Merrill Kelly | SP | Ari | 36 | |
| Seth Lugo | SP | KC | 35 | |
| Casey Mize | SP | Det | 28 | |
| Andre Pallante | SP | StL | 26 | |
| Martin Perez | SP | ChA | 34 | April 19, 2025:60 Day IL |
| David Peterson | SP | NYN | 29 | |
| Landen Roupp | SP | SF | N/A | |
| Shane Smith | SP | ChA | N/A | |
| Mason Miller | RP | Oak | 26 | |
| Andres Munoz | RP | Sea | 26 |