| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Samuel Basallo | C | Bal | 20 | |
| Shea Langeliers | C | Oak | 27 | |
| Agustin Ramirez | C | Mia | 23 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Ryan Mountcastle | 1B | Bal | 28 | |
| Travis Bazzana | 2B | Cle | 22 | |
| Luis Garcia | 2B | Was | 24 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Kyren Paris | SS (2B,OF) | LAA | 23 | |
| Trea Turner | SS | Phi | 31 | |
| Alec Bohm | 3B | Phi | 28 | |
| Caleb Durbin | 3B | Mil | 25 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Wilyer Abreu | OF | Bos | 25 | |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Roman Anthony | OF | Bos | 20 | |
| Jordan Beck | OF | Col | 24 | |
| Jackson Chourio | OF | Mil | 21 | |
| Lars Nootbaar | OF | StL | 27 | |
| Julio Rodriguez | OF | Sea | 24 | |
| Bubba Chandler | SP | Pit | N/A | |
| MacKenzie Gore | SP | Was | 26 | |
| Gunnar Hoglund | SP | Oak | 25 | |
| Jackson Jobe | SP | Det | 22 | |
| Jesus Luzardo | SP | Phi | 27 | |
| Dustin May | SP | LAD | 27 | |
| Shane McClanahan | SP | TB | 28 | March 26, 2025:60 Day IL-Ab strain |
| Lance McCullers | SP | Hou | 31 | March 27, 2025:15 Day IL |
| Andrew Painter | SP | Phi | N/A | |
| Freddy Peralta | SP | Mil | 28 | |
| Robbie Ray | SP | SF | 33 | |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| Paul Skenes | SP | Pit | 22 | |
| José Alvarado | RP | Phi | 29 |