| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Carson Kelly | C | ChN | 30 | |
| Edgar Quero | C | ChA | 22 | |
| Tyler Stephenson | C | Cin | 28 | March 26, 2025:10 Day IL |
| Jac Caglianone | 1B | KC | 22 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Matt Olson | 1B | Atl | 31 | |
| Eric Wagaman | 1B (OF) | Mia | N/A | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Jonathan India | 2B (3B,OF) | KC | 28 | |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Bo Bichette | SS | Tor | 27 | |
| Jacob Wilson | SS | Oak | 23 | |
| Noelvi Marte | 3B | Cin | 23 | |
| Austin Riley | 3B | Atl | 28 | |
| Matt Shaw | 3B | ChN | 23 | |
| Wilyer Abreu | OF | Bos | 25 | |
| Roman Anthony | OF | Bos | 20 | |
| Andrew Benintendi | OF | ChA | 30 | |
| Lawrence Butler | OF | Oak | 24 | |
| Oneil Cruz | OF | Pit | 26 | |
| Justyn-Henry Malloy | OF | Det | 25 | |
| Heliot Ramos | OF | SF | 25 | |
| Chandler Simpson | OF | TB | 24 | |
| Tyler Anderson | SP | LAA | 35 | |
| Spencer Arrighetti | SP | Hou | N/A | April 08, 2025:15 Day IL |
| Walker Buehler | SP | Bos | 30 | |
| Pat Corbin | SP | Tex | 35 | |
| Garrett Crochet | SP | Bos | 25 | |
| Max Meyer | SP | Mia | 26 | |
| Colin Rea | SP (RP) | ChN | 34 | |
| Kodai Senga | SP | NYN | 32 | |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Justin Verlander | SP | SF | 42 | |
| Edwin Diaz | RP | NYN | 31 |