| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Pedro Pages | C | StL | 26 | |
| Adley Rutschman | C | Bal | 27 | |
| Yandy Diaz | 1B | TB | 33 | |
| Paul Goldschmidt | 1B | NYA | 37 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Gleyber Torres | 2B | Det | 28 | |
| Javier Baez | SS (3B,OF) | Det | 32 | |
| Kyle Farmer | SS (2B) | Col | 34 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Ha-seong Kim | SS | TB | 29 | March 27, 2025:10 Day IL |
| JJ Wetherholt | SS | StL | 22 | |
| Masyn Winn | SS | StL | 23 | |
| Max Muncy | 3B | LAD | 34 | |
| Jackson Chourio | OF | Mil | 21 | |
| TJ Friedl | OF | Cin | 29 | |
| Michael Harris II | OF | Atl | 24 | |
| Brandon Nimmo | OF | NYN | 32 | |
| Lars Nootbaar | OF | StL | 27 | |
| George Springer | OF | Tor | 35 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Tim Tawa | OF (2B) | Ari | N/A | |
| Brayan Bello | SP | Bos | 25 | |
| Tanner Bibee | SP | Cle | 26 | |
| Hunter Brown | SP | Hou | 26 | |
| Corbin Burnes | SP | Ari | 30 | |
| Yu Darvish | SP | SD | 38 | March 27, 2025:15 Day IL |
| Zach Eflin | SP | Bal | 31 | April 09, 2025:15 Day IL |
| Logan Gilbert | SP | Sea | 27 | April 26, 2025:15 Day IL |
| Sonny Gray | SP | StL | 35 | |
| Dustin May | SP | LAD | 27 | |
| Bailey Ober | SP | Min | 29 | |
| Andre Pallante | SP | StL | 26 | |
| Clarke Schmidt | SP | NYA | 29 | |
| Trevor Williams | SP | Was | 33 |