| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Jonah Heim | C | Tex | 29 | |
| Alejandro Kirk | C | Tor | 26 | |
| Triston Casas | 1B | Bos | 25 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Michael Toglia | 1B | Col | 26 | |
| Matt McLain | 2B | Cin | 25 | |
| Brendan Rodgers | 2B | Hou | 28 | |
| Kyren Paris | SS (2B,OF) | LAA | 23 | |
| Jeremy Pena | SS | Hou | 27 | |
| Anthony Volpe | SS | NYA | 24 | |
| Josh Jung | 3B | Tex | 27 | |
| Jordan Westburg | 3B (2B) | Bal | 26 | April 28, 2025:10 Day IL |
| Corbin Carroll | OF | Ari | 24 | |
| Riley Greene | OF | Det | 24 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Andrew McCutchen | OF | Pit | 38 | |
| Jackson Merrill | OF | SD | 22 | April 08, 2025:10 Day IL |
| Spencer Steer | OF (1B) | Cin | 27 | |
| Jordan Walker | OF | StL | 22 | |
| Chase Burns | SP | Cin | 22 | |
| Richard Fitts | SP | Bos | N/A | April 13, 2025:15 Day IL |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Jack Leiter | SP | Tex | N/A | |
| Tylor Megill | SP | NYN | 29 | |
| Robbie Ray | SP | SF | 33 | |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| Landen Roupp | SP | SF | N/A | |
| Max Scherzer | SP | Tor | 40 | March 30, 2025:15 Day IL-Thumb |
| Spencer Schwellenbach | SP | Atl | 24 | |
| Bryan Woo | SP | Sea | 25 | |
| David Bednar | RP | Pit | 30 | |
| Carlos Estevez | RP | KC | 32 | |
| Mason Miller | RP | Oak | 26 | |
| Cade Smith | RP | Cle | N/A |