| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Drake Baldwin | C | Atl | 24 | |
| Ivan Herrera | C | StL | 24 | April 07, 2025:15 Day IL-Knee |
| Alejandro Kirk | C | Tor | 26 | |
| Jake Burger | 1B | Tex | 29 | |
| Deyvison De Los Santos | 1B | Mia | 21 | |
| Kyle Manzardo | 1B | Cle | 24 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Kristian Campbell | 2B (OF) | Bos | 22 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Gleyber Torres | 2B | Det | 28 | |
| Adael Amador | SS (2B) | Col | N/A | |
| Carlos Correa | SS | Min | 30 | |
| J.P. Crawford | SS | Sea | 30 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Noelvi Marte | 3B | Cin | 23 | |
| Max Muncy | 3B | LAD | 34 | |
| Colton Cowser | OF | Bal | 25 | March 31, 2025:60 Day IL-Left thumb fracture |
| Pete Crow-Armstrong | OF | ChN | 23 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Lars Nootbaar | OF | StL | 27 | |
| Chandler Simpson | OF | TB | 24 | |
| Mike Yastrzemski | OF | SF | 34 | |
| Andrew Abbott | SP | Cin | 25 | |
| Sandy Alcantara | SP | Mia | 29 | |
| Osvaldo Bido | SP | Oak | N/A | |
| Griffin Canning | SP | NYN | 28 | |
| Tylor Megill | SP | NYN | 29 | |
| Andrew Painter | SP | Phi | N/A | |
| Kumar Rocker | SP | Tex | N/A | April 25, 2025:15 Day IL |
| Landen Roupp | SP | SF | N/A | |
| Spencer Schwellenbach | SP | Atl | 24 | |
| Jeffrey Springs | SP | Oak | 32 | |
| Alexis Diaz | RP | Cin | 28 | |
| Ryan Helsley | RP | StL | 30 |