| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Jonah Heim | C | Tex | 29 | |
| Will Smith | C | LAD | 30 | |
| Michael Busch | 1B | ChN | 27 | |
| Jac Caglianone | 1B | KC | 22 | |
| Nolan Schanuel | 1B | LAA | 23 | |
| Luis Garcia | 2B | Was | 24 | |
| Brooks Lee | 2B (3B) | Min | 24 | |
| Brice Turang | 2B | Mil | 25 | |
| CJ Abrams | SS | Was | 24 | |
| Carson Williams | SS | TB | 21 | |
| Bobby Witt Jr. | SS | KC | 24 | |
| Alex Bregman | 3B | Bos | 31 | |
| Matt Shaw | 3B | ChN | 23 | |
| Ben Williamson | 3B | Sea | N/A | |
| Brenton Doyle | OF | Col | 26 | |
| Michael Harris II | OF | Atl | 24 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Ceddanne Rafaela | OF | Bos | 24 | |
| Victor Scott | OF | StL | 24 | |
| Jorge Soler | OF | LAA | 32 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Andrew Abbott | SP | Cin | 25 | |
| Taj Bradley | SP | TB | 24 | |
| Max Fried | SP | NYA | 31 | |
| Zac Gallen | SP | Ari | 29 | |
| Logan Gilbert | SP | Sea | 27 | April 26, 2025:15 Day IL |
| Sonny Gray | SP | StL | 35 | |
| Mitch Keller | SP | Pit | 29 | |
| Jack Leiter | SP | Tex | N/A | |
| Jeffrey Springs | SP | Oak | 32 | |
| Logan Webb | SP | SF | 28 | |
| Emmanuel Clase | RP | Cle | 27 | |
| Tanner Scott | RP | LAD | 30 |