| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Drake Baldwin | C | Atl | 24 | |
| Ryan Jeffers | C | Min | 27 | |
| Triston Casas | 1B | Bos | 25 | |
| Vinnie Pasquantino | 1B | KC | 27 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Andrés Gimenez | 2B | Tor | 26 | |
| Nick Gonzales | 2B | Pit | 25 | March 28, 2025:10 Day IL |
| Matt McLain | 2B | Cin | 25 | |
| Gabriel Arias | SS (2B) | Cle | 25 | |
| Masyn Winn | SS | StL | 23 | |
| Rafael Devers | 3B | Bos | 28 | |
| Chase Meidroth | 3B (2B,SS) | ChA | N/A | |
| Dylan Moore | 3B (2B,OF) | Sea | 32 | April 29, 2025:10 Day IL |
| Eugenio Suarez | 3B | Ari | 33 | |
| Lawrence Butler | OF | Oak | 24 | |
| Trent Grisham | OF | NYA | 28 | |
| Michael Harris II | OF | Atl | 24 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Julio Rodriguez | OF | Sea | 24 | |
| Spencer Steer | OF (1B) | Cin | 27 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Jordan Walker | OF | StL | 22 | |
| Jose Berrios | SP | Tor | 30 | |
| Taj Bradley | SP | TB | 24 | |
| Erick Fedde | SP | StL | 32 | |
| Zack Littell | SP | TB | 29 | |
| Casey Mize | SP | Det | 28 | |
| Colin Rea | SP (RP) | ChN | 34 | |
| Shane Smith | SP | ChA | N/A | |
| Jeffrey Springs | SP | Oak | 32 | |
| Logan Webb | SP | SF | 28 | |
| Edwin Diaz | RP | NYN | 31 | |
| Ryan Helsley | RP | StL | 30 |