| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Miguel Amaya | C | ChN | 26 | |
| Alejandro Kirk | C | Tor | 26 | |
| Rhys Hoskins | 1B | Mil | 32 | |
| Colt Keith | 1B (2B) | Det | 23 | |
| Matt Mervis | 1B | Mia | 27 | |
| Spencer Torkelson | 1B | Det | 25 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Matt McLain | 2B | Cin | 25 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Ezequiel Tovar | SS | Col | 23 | April 19, 2025:10 Day IL |
| Alec Bohm | 3B | Phi | 28 | |
| Max Muncy | 3B | LAD | 34 | |
| Ben Williamson | 3B | Sea | N/A | |
| Nick Castellanos | OF | Phi | 33 | |
| Trent Grisham | OF | NYA | 28 | |
| Austin Hays | OF | Cin | 29 | May 02, 2025:10 Day IL |
| Bryan Reynolds | OF | Pit | 30 | |
| Spencer Steer | OF (1B) | Cin | 27 | |
| Tim Tawa | OF (2B) | Ari | N/A | |
| Mike Yastrzemski | OF | SF | 34 | |
| Matt Boyd | SP | ChN | 34 | |
| Hunter Brown | SP | Hou | 26 | |
| Nestor Cortes Jr. | SP | Mil | 30 | April 06, 2025:60 Day IL-Left elbow flexor strain |
| Hunter Dobbins | SP | Bos | N/A | |
| Erick Fedde | SP | StL | 32 | |
| Casey Mize | SP | Det | 28 | |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| Paul Skenes | SP | Pit | 22 | |
| Tarik Skubal | SP | Det | 28 | |
| Jeffrey Springs | SP | Oak | 32 | |
| Felix Bautista | RP | Bal | 29 | |
| Emmanuel Clase | RP | Cle | 27 | |
| Camilo Doval | RP | SF | 27 |