| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Sean Murphy | C | Atl | 30 | |
| Agustin Ramirez | C | Mia | 23 | |
| Austin Wells | C | NYA | 25 | |
| Jac Caglianone | 1B | KC | 22 | |
| Vladimir Guerrero Jr. | 1B | Tor | 26 | |
| Nick Kurtz | 1B | Oak | 22 | |
| Spencer Torkelson | 1B | Det | 25 | |
| Travis Bazzana | 2B | Cle | 22 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Sebastian Walcott | SS | Tex | 19 | |
| Caleb Durbin | 3B | Mil | 25 | |
| Cam Smith | 3B (OF) | Hou | 22 | |
| Jordan Westburg | 3B (2B) | Bal | 26 | April 28, 2025:10 Day IL |
| Corbin Carroll | OF | Ari | 24 | |
| Tommy Edman | OF (2B) | LAD | 29 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Jackson Merrill | OF | SD | 22 | April 08, 2025:10 Day IL |
| Julio Rodriguez | OF | Sea | 24 | |
| Shohei Ohtani | DH ,SP | LAD | 30 | |
| Logan Gilbert | SP | Sea | 27 | April 26, 2025:15 Day IL |
| Gunnar Hoglund | SP | Oak | 25 | |
| Clay Holmes | SP | NYN | 32 | |
| Grant Holmes | SP | Atl | N/A | |
| Cade Horton | SP | ChN | 23 | |
| Jackson Jobe | SP | Det | 22 | |
| Michael King | SP | SD | 29 | |
| Jack Leiter | SP | Tex | N/A | |
| Dustin May | SP | LAD | 27 | |
| Cole Ragans | SP | KC | 27 | |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Aroldis Chapman | RP | Bos | 37 | |
| Jeff Hoffman | RP | Tor | 32 |