| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Samuel Basallo | C | Bal | 20 | |
| J.T. Realmuto | C | Phi | 34 | |
| Dalton Rushing | C | LAD | 24 | |
| Kyle Teel | C | ChA | 23 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| LaMonte Wade Jr | 1B | SF | 31 | |
| Brendan Donovan | 2B (SS,OF) | StL | 28 | |
| Ketel Marte | 2B | Ari | 31 | April 05, 2025:10 Day IL |
| Bryson Stott | 2B | Phi | 27 | |
| Carlos Correa | SS | Min | 30 | |
| Jacob Wilson | SS | Oak | 23 | |
| Jace Jung | 3B | Det | 24 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Coby Mayo | 3B | Bal | 23 | |
| Miguel Vargas | 3B | ChA | 25 | |
| Kerry Carpenter | OF | Det | 27 | |
| Evan Carter | OF | Tex | 22 | |
| Dylan Crews | OF | Was | 23 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Lars Nootbaar | OF | StL | 27 | |
| Anthony Santander | OF | Tor | 30 | |
| Zac Veen | OF | Col | N/A | |
| Mike Yastrzemski | OF | SF | 34 | |
| Chase Dollander | SP | Col | N/A | |
| Tony Gonsolin | SP | LAD | 30 | |
| Zebby Matthews | SP | Min | N/A | |
| Andrew Painter | SP | Phi | N/A | |
| Mitchell Parker | SP | Was | 25 | |
| Jeffrey Springs | SP | Oak | 32 | |
| Ranger Suarez | SP | Phi | 29 | March 23, 2025:15 Day IL |
| Randy Vasquez | SP | SD | 26 | |
| Bryan Woo | SP | Sea | 25 | |
| Alexis Diaz | RP | Cin | 28 | |
| Carlos Estevez | RP | KC | 32 |