| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Drake Baldwin | C | Atl | 24 | |
| Adley Rutschman | C | Bal | 27 | |
| Michael Busch | 1B | ChN | 27 | |
| Nathaniel Lowe | 1B | Was | 29 | |
| Jose Altuve | 2B (OF) | Hou | 34 | |
| Nico Hoerner | 2B | ChN | 27 | |
| Brooks Lee | 2B (3B) | Min | 24 | |
| Xander Bogaerts | SS | SD | 32 | |
| Carlos Correa | SS | Min | 30 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Matt Chapman | 3B | SF | 32 | |
| Matt Vierling | 3B | Det | 28 | March 27, 2025:10 Day IL |
| Hunter Goodman | OF (C) | Col | 25 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Heliot Ramos | OF | SF | 25 | |
| Pavin Smith | OF (1B) | Ari | 29 | |
| Fernando Tatis Jr. | OF | SD | 26 | |
| Matt Wallner | OF | Min | 27 | April 17, 2025:10 Day IL |
| Taylor Ward | OF | LAA | 31 | |
| Logan Allen | SP | Cle | 26 | |
| Osvaldo Bido | SP | Oak | N/A | |
| Gerrit Cole | SP | NYA | 34 | March 22, 2025:60 Day IL |
| Yu Darvish | SP | SD | 38 | March 27, 2025:15 Day IL |
| Max Fried | SP | NYA | 31 | |
| Andrew Heaney | SP | Pit | 33 | |
| Shota Imanaga | SP | ChN | 31 | |
| Lance McCullers | SP | Hou | 31 | March 27, 2025:15 Day IL |
| Tobias Myers | SP | Mil | N/A | |
| Jose Quintana | SP | Mil | 36 | |
| Landen Roupp | SP | SF | N/A | |
| Clarke Schmidt | SP | NYA | 29 | |
| Spencer Schwellenbach | SP | Atl | 24 | |
| Trevor Megill | RP | Mil | 31 |