| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| J.T. Realmuto | C | Phi | 34 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Vladimir Guerrero Jr. | 1B | Tor | 26 | |
| Kyle Manzardo | 1B | Cle | 24 | |
| Eric Wagaman | 1B (OF) | Mia | N/A | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Gavin Lux | 2B (OF) | Cin | 27 | |
| Willy Adames | SS | SF | 29 | |
| Jeremy Pena | SS | Hou | 27 | |
| Manny Machado | 3B | SD | 32 | |
| Mark Vientos | 3B | NYN | 25 | |
| JJ Bleday | OF | Oak | 27 | |
| Heston Kjerstad | OF | Bal | 26 | |
| Justyn-Henry Malloy | OF | Det | 25 | |
| Kameron Misner | OF | TB | N/A | |
| Andy Pages | OF | LAD | 24 | |
| Luis Robert Jr. | OF | ChA | 27 | |
| Brent Rooker | OF | Oak | 30 | |
| Kyle Stowers | OF | Mia | 27 | |
| Tim Tawa | OF (2B) | Ari | N/A | |
| Daulton Varsho | OF | Tor | 28 | |
| Sandy Alcantara | SP | Mia | 29 | |
| Chris Bassitt | SP | Tor | 36 | |
| Shane Baz | SP | TB | 25 | |
| Gerrit Cole | SP | NYA | 34 | March 22, 2025:60 Day IL |
| Jacob deGrom | SP | Tex | 36 | |
| Andrew Heaney | SP | Pit | 33 | |
| Mitch Keller | SP | Pit | 29 | |
| Matthew Liberatore | SP | StL | 25 | |
| Zebby Matthews | SP | Min | N/A | |
| José Soriano | SP | LAA | 26 | |
| Bryan Woo | SP | Sea | 25 | |
| Camilo Doval | RP | SF | 27 | |
| Tanner Scott | RP | LAD | 30 |