| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Samuel Basallo | C | Bal | 20 | |
| Carson Kelly | C | ChN | 30 | |
| Will Smith | C | LAD | 30 | |
| Ryan Mountcastle | 1B | Bal | 28 | |
| Vinnie Pasquantino | 1B | KC | 27 | |
| Wilmer Flores | 2B | SF | 33 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Otto Lopez | 2B | Mia | 26 | |
| Carlos Correa | SS | Min | 30 | |
| Trea Turner | SS | Phi | 31 | |
| Alex Bregman | 3B | Bos | 31 | |
| Kerry Carpenter | OF | Det | 27 | |
| Adolis Garcia | OF | Tex | 32 | |
| Michael Harris II | OF | Atl | 24 | |
| Austin Hays | OF | Cin | 29 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Heliot Ramos | OF | SF | 25 | |
| Victor Scott | OF | StL | 24 | |
| Chandler Simpson | OF | TB | 24 | |
| James Wood | OF | Was | 22 | |
| Taj Bradley | SP | TB | 24 | |
| Chase Dollander | SP | Col | N/A | |
| Erick Fedde | SP | StL | 32 | |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Kyle Hendricks | SP | LAA | 35 | |
| Tylor Megill | SP | NYN | 29 | |
| Max Meyer | SP | Mia | 26 | |
| Carlos Rodon | SP | NYA | 32 | |
| Eduardo Rodriguez | SP | Ari | 32 | |
| Jameson Taillon | SP | ChN | 33 | |
| Ryan Weathers | SP | Mia | 25 | March 27, 2025:15 Day IL |
| Luke Jackson | RP | Tex | 33 | |
| Emilio Pagan | RP | Cin | 33 |