| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Drake Baldwin | C | Atl | 24 | |
| Dillon Dingler | C | Det | N/A | |
| Triston Casas | 1B | Bos | 25 | May 03, 2025:10 Day IL |
| Matt Mervis | 1B | Mia | 27 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Kristian Campbell | 2B (OF) | Bos | 22 | |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Brooks Lee | 2B (3B) | Min | 24 | |
| Gleyber Torres | 2B | Det | 28 | |
| Xander Bogaerts | SS | SD | 32 | |
| J.P. Crawford | SS | Sea | 30 | |
| Ezequiel Tovar | SS | Col | 23 | April 19, 2025:10 Day IL |
| Connor Norby | 3B | Mia | 24 | |
| Jorge Polanco | 3B | Sea | 31 | |
| Matt Shaw | 3B | ChN | 23 | |
| Roman Anthony | OF | Bos | 20 | |
| Byron Buxton | OF | Min | 31 | |
| Jasson Dominguez | OF | NYA | 22 | |
| Sal Frelick | OF | Mil | 25 | |
| Andrew Abbott | SP | Cin | 25 | |
| Brayan Bello | SP | Bos | 25 | |
| Chase Burns | SP | Cin | 22 | |
| Garrett Crochet | SP | Bos | 25 | |
| Lucas Giolito | SP | Bos | 30 | |
| Tanner Houck | SP | Bos | 28 | |
| Nick Lodolo | SP | Cin | 27 | |
| Pablo Lopez | SP | Min | 29 | |
| Brandon Pfaadt | SP | Ari | 26 | |
| Shane Smith | SP | ChA | N/A | |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Kenley Jansen | RP | LAA | 37 | |
| Ryan Pressly | RP | ChN | 36 |