| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Joey Bart | C | Pit | 28 | |
| Jonah Heim | C | Tex | 29 | |
| Will Smith | C | LAD | 30 | |
| Austin Wynns | C | Cin | 34 | |
| Willson Contreras | 1B | StL | 32 | |
| Yandy Diaz | 1B | TB | 33 | |
| Jose Altuve | 2B (OF) | Hou | 34 | |
| Romy Gonzalez | 2B (1B) | Bos | 28 | |
| Luis Rengifo | 2B (3B) | LAA | 28 | |
| CJ Abrams | SS | Was | 24 | |
| Xavier Edwards | SS | Mia | 25 | |
| Matt Chapman | 3B | SF | 32 | |
| Josh Smith | 3B (SS) | Tex | 27 | |
| JJ Bleday | OF | Oak | 27 | |
| Lawrence Butler | OF | Oak | 24 | |
| TJ Friedl | OF | Cin | 29 | |
| Brandon Nimmo | OF | NYN | 32 | |
| Ceddanne Rafaela | OF | Bos | 24 | |
| Brent Rooker | OF | Oak | 30 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Mike Yastrzemski | OF | SF | 34 | |
| Chris Bassitt | SP | Tor | 36 | |
| Jose Berrios | SP | Tor | 30 | |
| Osvaldo Bido | SP | Oak | N/A | |
| Erick Fedde | SP | StL | 32 | |
| Jack Flaherty | SP | Det | 29 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Sean Manaea | SP | NYN | 33 | March 27, 2025:60 Day IL |
| Kodai Senga | SP | NYN | 32 | |
| Justin Steele | SP | ChN | 29 | April 09, 2025:60 Day IL |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Josh Hader | RP | Hou | 31 | |
| Cade Smith | RP | Cle | N/A | |
| Taijuan Walker | RP (SP) | Phi | 32 |