| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Adley Rutschman | C | Bal | 27 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Vinnie Pasquantino | 1B | KC | 27 | |
| Ben Rice | 1B | NYA | 26 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Bryson Stott | 2B | Phi | 27 | |
| Xander Bogaerts | SS | SD | 32 | |
| Jose Caballero | SS (3B,OF) | TB | 28 | |
| Xavier Edwards | SS | Mia | 25 | |
| Brady House | 3B | Was | 21 | |
| Josh Jung | 3B | Tex | 27 | |
| Matt Shaw | 3B | ChN | 23 | |
| Pete Crow-Armstrong | OF | ChN | 23 | |
| Adolis Garcia | OF | Tex | 32 | |
| Riley Greene | OF | Det | 24 | |
| Austin Hays | OF | Cin | 29 | |
| Brandon Nimmo | OF | NYN | 32 | |
| Victor Scott | OF | StL | 24 | |
| Kyle Stowers | OF | Mia | 27 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Zac Veen | OF | Col | N/A | |
| Andrew Abbott | SP | Cin | 25 | |
| Walker Buehler | SP | Bos | 30 | |
| Corbin Burnes | SP | Ari | 30 | |
| Bubba Chandler | SP | Pit | N/A | |
| Jackson Jobe | SP | Det | 22 | |
| Freddy Peralta | SP | Mil | 28 | |
| David Peterson | SP | NYN | 29 | |
| Eduardo Rodriguez | SP | Ari | 32 | |
| Max Scherzer | SP | Tor | 40 | March 30, 2025:15 Day IL-Thumb |
| Tarik Skubal | SP | Det | 28 | |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Gavin Williams | SP | Cle | 25 |