| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Francisco Álvarez | C | NYN | 23 | |
| Miguel Amaya | C | ChN | 26 | |
| Tyler Stephenson | C | Cin | 28 | |
| Nathaniel Lowe | 1B | Was | 29 | |
| Matt Mervis | 1B | Mia | 27 | |
| Spencer Torkelson | 1B | Det | 25 | |
| Christian Walker | 1B | Hou | 34 | |
| Ozzie Albies | 2B | Atl | 28 | |
| Luis Rengifo | 2B (3B) | LAA | 28 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Rafael Devers | 3B | Bos | 28 | |
| Max Muncy | 3B | LAD | 34 | |
| Jordan Beck | OF | Col | 24 | |
| Bryan Reynolds | OF | Pit | 30 | |
| Brent Rooker | OF | Oak | 30 | |
| Kyle Schwarber | OF | Phi | 32 | |
| Kyle Tucker | OF | ChN | 28 | |
| Shane Baz | SP | TB | 25 | |
| Jose Berrios | SP | Tor | 30 | |
| Matt Boyd | SP | ChN | 34 | |
| Nestor Cortes Jr. | SP | Mil | 30 | April 06, 2025:60 Day IL-Left elbow flexor strain |
| Hunter Dobbins | SP | Bos | N/A | |
| Max Fried | SP | NYA | 31 | |
| Nick Lodolo | SP | Cin | 27 | |
| Jesus Luzardo | SP | Phi | 27 | |
| Casey Mize | SP | Det | 28 | |
| Chris Sale | SP | Atl | 36 | |
| Brady Singer | SP | Cin | 28 | |
| Shane Smith | SP | ChA | N/A | |
| Felix Bautista | RP | Bal | 29 | |
| Ryan Pressly | RP | ChN | 36 | |
| Ryan Walker | RP | SF | N/A |