| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Cal Raleigh | C | Sea | 28 | |
| Austin Wells | C | NYA | 25 | |
| Jake Burger | 1B | Tex | 29 | |
| Jac Caglianone | 1B | KC | 22 | |
| Spencer Torkelson | 1B | Det | 25 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Brice Turang | 2B | Mil | 25 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Zach Neto | SS | LAA | 24 | |
| Anthony Volpe | SS | NYA | 24 | |
| Masyn Winn | SS | StL | 23 | |
| Andy Ibáñez | 3B | Det | 32 | |
| Chase Meidroth | 3B (2B,SS) | ChA | N/A | |
| Jose Ramirez | 3B | Cle | 32 | |
| Matt Shaw | 3B | ChN | 23 | |
| Yordan Alvarez | OF | Hou | 27 | |
| Jackson Chourio | OF | Mil | 21 | |
| Lourdes Gurriel | OF | Ari | 31 | |
| Jackson Merrill | OF | SD | 22 | April 08, 2025:10 Day IL |
| Garrett Mitchell | OF | Mil | 26 | April 26, 2025:10 Day IL |
| Seiya Suzuki | OF | ChN | 30 | |
| Logan Allen | SP | Cle | 26 | |
| Spencer Arrighetti | SP | Hou | N/A | April 08, 2025:15 Day IL |
| Garrett Crochet | SP | Bos | 25 | |
| Jack Flaherty | SP | Det | 29 | |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Nick Lodolo | SP | Cin | 27 | |
| Bryce Miller | SP | Sea | 26 | |
| Jameson Taillon | SP | ChN | 33 | |
| Logan Webb | SP | SF | 28 | |
| Pete Fairbanks | RP | TB | 31 | |
| Tanner Scott | RP | LAD | 30 | |
| Cade Smith | RP | Cle | N/A | |
| Ryan Walker | RP | SF | N/A |