| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Yainer Diaz | C | Hou | 26 | |
| Austin Wells | C | NYA | 25 | |
| Jonathan Aranda | 1B | TB | 26 | |
| Vladimir Guerrero Jr. | 1B | Tor | 26 | |
| Nick Kurtz | 1B | Oak | 22 | |
| Eric Wagaman | 1B (OF) | Mia | N/A | |
| Wilmer Flores | 2B | SF | 33 | |
| Matt McLain | 2B | Cin | 25 | |
| Jeff McNeil | 2B | NYN | 33 | |
| Xander Bogaerts | SS | SD | 32 | |
| Trevor Story | SS | Bos | 32 | |
| Trea Turner | SS | Phi | 31 | |
| Rafael Devers | 3B | Bos | 28 | |
| Cam Smith | 3B (OF) | Hou | 22 | |
| Eugenio Suarez | 3B | Ari | 33 | |
| Jasson Dominguez | OF | NYA | 22 | |
| Sal Frelick | OF | Mil | 25 | |
| Riley Greene | OF | Det | 24 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Heliot Ramos | OF | SF | 25 | |
| Shane Bieber | SP | Cle | 29 | March 27, 2025:15 Day IL |
| Jacob deGrom | SP | Tex | 36 | |
| Zac Gallen | SP | Ari | 29 | |
| Lucas Giolito | SP | Bos | 30 | |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Lance McCullers | SP | Hou | 31 | March 27, 2025:15 Day IL |
| Ryan Pepiot | SP | TB | 27 | |
| Robbie Ray | SP | SF | 33 | |
| Chris Sale | SP | Atl | 36 | |
| Cristopher Sanchez | SP | Phi | 28 | |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Josh Hader | RP | Hou | 31 | |
| Devin Williams | RP | NYA | 30 |