| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Dillon Dingler | C | Det | N/A | |
| Bo Naylor | C | Cle | 25 | |
| Jose Trevino | C | Cin | 32 | |
| Spencer Horwitz | 1B | Pit | 27 | March 27, 2025:10 Day IL |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Willi Castro | 2B (SS,3B) | Min | 28 | April 24, 2025:10 Day IL |
| Jake Cronenworth | 2B | SD | 31 | April 11, 2025:10 Day IL |
| Bryson Stott | 2B | Phi | 27 | |
| Carlos Correa | SS | Min | 30 | |
| Paul DeJong | SS (3B) | Was | 31 | April 16, 2025:10 Day IL-Fractured nose |
| Ernie Clement | 3B | Tor | 29 | |
| Max Muncy | 3B | LAD | 34 | |
| Jose Ramirez | 3B | Cle | 32 | |
| Alec Burleson | OF (1B) | StL | 26 | |
| Kerry Carpenter | OF | Det | 27 | |
| Corbin Carroll | OF | Ari | 24 | |
| Steven Kwan | OF | Cle | 27 | |
| Cedric Mullins II | OF | Bal | 30 | |
| Kyle Tucker | OF | ChN | 28 | |
| Jordan Walker | OF | StL | 22 | |
| Andrew Abbott | SP | Cin | 25 | |
| Logan Allen | SP | Cle | 26 | |
| Osvaldo Bido | SP | Oak | N/A | |
| Luis Castillo | SP | Sea | 32 | |
| Yu Darvish | SP | SD | 38 | March 27, 2025:15 Day IL |
| Erick Fedde | SP | StL | 32 | |
| Jake Irvin | SP | Was | 28 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Pablo Lopez | SP | Min | 29 | |
| Nick Pivetta | SP | SD | 32 | |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| JP Sears | SP | Oak | 29 | |
| José Soriano | SP | LAA | 26 | |
| Zack Wheeler | SP | Phi | 34 |