| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Edgar Quero | C | ChA | 22 | |
| Agustin Ramirez | C | Mia | 23 | |
| Adley Rutschman | C | Bal | 27 | |
| Triston Casas | 1B | Bos | 25 | |
| C.J. Kayfus | 1B | Cle | 23 | |
| Matt Olson | 1B | Atl | 31 | |
| Kristian Campbell | 2B (OF) | Bos | 22 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Jacob Wilson | SS | Oak | 23 | |
| Austin Riley | 3B | Atl | 28 | |
| Mark Vientos | 3B | NYN | 25 | |
| Lawrence Butler | OF | Oak | 24 | |
| Jackson Chourio | OF | Mil | 21 | |
| Dylan Crews | OF | Was | 23 | |
| Oneil Cruz | OF | Pit | 26 | |
| Trent Grisham | OF | NYA | 28 | |
| Wyatt Langford | OF | Tex | 23 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Chandler Simpson | OF | TB | 24 | |
| Kris Bubic | SP | KC | 27 | |
| Dylan Cease | SP | SD | 29 | |
| Logan Gilbert | SP | Sea | 27 | April 26, 2025:15 Day IL |
| Tony Gonsolin | SP | LAD | 30 | |
| Ryan Gusto | SP (RP) | Hou | N/A | |
| Gunnar Hoglund | SP | Oak | 25 | |
| Zebby Matthews | SP | Min | N/A | |
| Shane McClanahan | SP | TB | 28 | March 26, 2025:60 Day IL-Ab strain |
| Tylor Megill | SP | NYN | 29 | |
| Bryce Miller | SP | Sea | 26 | |
| Casey Mize | SP | Det | 28 | |
| Freddy Peralta | SP | Mil | 28 | |
| Landen Roupp | SP | SF | N/A | |
| Mason Miller | RP | Oak | 26 | |
| Andres Munoz | RP | Sea | 26 |