| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Jonah Heim | C | Tex | 29 | |
| Agustin Ramirez | C | Mia | 23 | |
| Yandy Diaz | 1B | TB | 33 | |
| Christian Walker | 1B | Hou | 34 | |
| Matt McLain | 2B | Cin | 25 | |
| J.P. Crawford | SS | Sea | 30 | |
| Zach Neto | SS | LAA | 24 | |
| Masyn Winn | SS | StL | 23 | |
| Josh Jung | 3B | Tex | 27 | |
| Isaac Paredes | 3B | Hou | 26 | |
| Josh Smith | 3B (SS) | Tex | 27 | |
| Lawrence Butler | OF | Oak | 24 | |
| Tommy Edman | OF (2B) | LAD | 29 | |
| Trent Grisham | OF | NYA | 28 | |
| Kameron Misner | OF | TB | N/A | |
| Brandon Nimmo | OF | NYN | 32 | |
| Andy Pages | OF | LAD | 24 | |
| Ceddanne Rafaela | OF | Bos | 24 | |
| Kyle Stowers | OF | Mia | 27 | |
| Andrew Abbott | SP | Cin | 25 | |
| Ronel Blanco | SP | Hou | 31 | |
| Erick Fedde | SP | StL | 32 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Nick Lodolo | SP | Cin | 27 | |
| Mitchell Parker | SP | Was | 25 | |
| José Soriano | SP | LAA | 26 | |
| Jeffrey Springs | SP | Oak | 32 | |
| Justin Steele | SP | ChN | 29 | April 09, 2025:60 Day IL |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Brandon Woodruff | SP | Mil | 32 | March 27, 2025:15 Day IL |
| Emmanuel Clase | RP | Cle | 27 | |
| Josh Hader | RP | Hou | 31 | |
| Cade Smith | RP | Cle | N/A | |
| Taijuan Walker | RP (SP) | Phi | 32 |