| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Dillon Dingler | C | Det | N/A | |
| Cal Raleigh | C | Sea | 28 | |
| Jac Caglianone | 1B | KC | 22 | |
| Matt Olson | 1B | Atl | 31 | |
| Ben Rice | 1B | NYA | 26 | |
| Otto Lopez | 2B | Mia | 26 | |
| Brandon Lowe | 2B | TB | 30 | |
| Christian Moore | 2B | LAA | 22 | |
| Gabriel Arias | SS (2B,3B) | Cle | 25 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Bobby Witt Jr. | SS | KC | 24 | |
| Coby Mayo | 3B | Bal | 23 | |
| Ryan McMahon | 3B | Col | 30 | |
| Eugenio Suarez | 3B | Ari | 33 | |
| Kerry Carpenter | OF | Det | 27 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Julio Rodriguez | OF | Sea | 24 | |
| Juan Soto | OF | NYN | 26 | |
| James Wood | OF | Was | 22 | |
| Shohei Ohtani | DH ,SP | LAD | 30 | |
| Spencer Arrighetti | SP | Hou | N/A | April 08, 2025:15 Day IL |
| Kris Bubic | SP | KC | 27 | |
| Logan Gilbert | SP | Sea | 27 | April 26, 2025:15 Day IL |
| Tony Gonsolin | SP | LAD | 30 | |
| MacKenzie Gore | SP | Was | 26 | |
| Nick Lodolo | SP | Cin | 27 | |
| Zebby Matthews | SP | Min | N/A | |
| Reese Olson | SP | Det | 25 | |
| Nick Pivetta | SP | SD | 32 | |
| Joe Ryan | SP | Min | 28 | |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Brandon Woodruff | SP | Mil | 32 | March 27, 2025:15 Day IL |
| Jeff Hoffman | RP | Tor | 32 | |
| Andres Munoz | RP | Sea | 26 |