| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Drake Baldwin | C | Atl | 24 | |
| Yainer Diaz | C | Hou | 26 | |
| Logan O'Hoppe | C | LAA | 25 | |
| Ryan Mountcastle | 1B | Bal | 28 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Ozzie Albies | 2B | Atl | 28 | |
| Angel Martinez | 2B (OF) | Cle | 23 | |
| CJ Abrams | SS | Was | 24 | |
| Zach Neto | SS | LAA | 24 | |
| Jacob Wilson | SS | Oak | 23 | |
| Alex Bregman | 3B | Bos | 31 | |
| Maikel Garcia | 3B (2B,OF) | KC | 25 | |
| Austin Riley | 3B | Atl | 28 | |
| Wilyer Abreu | OF | Bos | 25 | |
| Alec Burleson | OF (1B) | StL | 26 | |
| Jackson Chourio | OF | Mil | 21 | |
| Jarren Duran | OF | Bos | 28 | |
| TJ Friedl | OF | Cin | 29 | |
| Ceddanne Rafaela | OF | Bos | 24 | |
| Brayan Bello | SP | Bos | 25 | |
| Tanner Bibee | SP | Cle | 26 | |
| Shane Bieber | SP | Cle | 29 | March 27, 2025:15 Day IL |
| Corbin Burnes | SP | Ari | 30 | |
| Jack Flaherty | SP | Det | 29 | |
| Sonny Gray | SP | StL | 35 | |
| Rhett Lowder | SP | Cin | N/A | March 26, 2025:15 Day IL |
| Andre Pallante | SP | StL | 26 | |
| Freddy Peralta | SP | Mil | 28 | |
| Cole Ragans | SP | KC | 27 | |
| Tarik Skubal | SP | Det | 28 | |
| Josh Hader | RP | Hou | 31 | |
| Justin Martinez | RP | Ari | 23 | May 01, 2025:15 Day IL |
| Devin Williams | RP | NYA | 30 |