| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Dillon Dingler | C | Det | N/A | |
| Adley Rutschman | C | Bal | 27 | |
| Connor Wong | C | Bos | 28 | |
| Carlos Santana | 1B | Cle | 39 | |
| Andrew Vaughn | 1B | ChA | 27 | |
| Christian Walker | 1B | Hou | 34 | |
| Jose Altuve | 2B (OF) | Hou | 34 | |
| Brice Turang | 2B | Mil | 25 | |
| J.P. Crawford | SS | Sea | 30 | |
| Corey Seager | SS | Tex | 31 | April 23, 2025:10 Day IL |
| Masyn Winn | SS | StL | 23 | |
| Matt Chapman | 3B | SF | 32 | |
| Maikel Garcia | 3B (2B,OF) | KC | 25 | |
| Eugenio Suarez | 3B | Ari | 33 | |
| Wilyer Abreu | OF | Bos | 25 | |
| Lourdes Gurriel | OF | Ari | 31 | |
| Ian Happ | OF | ChN | 30 | |
| Teoscar Hernández | OF | LAD | 32 | |
| Jorge Soler | OF | LAA | 32 | |
| Kyle Stowers | OF | Mia | 27 | |
| Christian Yelich | OF | Mil | 33 | |
| Marcell Ozuna | DH | Atl | 34 | |
| Tyler Anderson | SP | LAA | 35 | |
| Nestor Cortes Jr. | SP | Mil | 30 | April 06, 2025:60 Day IL-Left elbow flexor strain |
| Nathan Eovaldi | SP | Tex | 35 | |
| Tony Gonsolin | SP | LAD | 30 | |
| Shota Imanaga | SP | ChN | 31 | |
| Merrill Kelly | SP | Ari | 36 | |
| Yusei Kikuchi | SP | LAA | 33 | |
| Ben Lively | SP | Cle | 33 | |
| Brady Singer | SP | Cin | 28 | |
| Pete Fairbanks | RP | TB | 31 | |
| Kenley Jansen | RP | LAA | 37 | |
| Tommy Kahnle | RP | Det | 35 |