| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Cal Raleigh | C | Sea | 28 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Rhys Hoskins | 1B | Mil | 32 | |
| Nathaniel Lowe | 1B | Was | 29 | |
| Ben Rice | 1B | NYA | 26 | |
| Travis Bazzana | 2B | Cle | 22 | |
| Nolan Gorman | 2B | StL | 24 | |
| Brandon Lowe | 2B | TB | 30 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Amed Rosario | SS (2B,3B) | Was | 29 | |
| Coby Mayo | 3B | Bal | 23 | |
| Ryan McMahon | 3B | Col | 30 | |
| Yoan Moncada | 3B | LAA | 29 | April 10, 2025:10 Day IL |
| Ben Williamson | 3B | Sea | N/A | |
| Jake Fraley | OF | Cin | 29 | |
| Hunter Goodman | OF (C) | Col | 25 | |
| Riley Greene | OF | Det | 24 | |
| Teoscar Hernández | OF | LAD | 32 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Juan Soto | OF | NYN | 26 | |
| Matt Wallner | OF | Min | 27 | April 17, 2025:10 Day IL |
| Eli White | OF | Atl | 30 | |
| Matt Boyd | SP | ChN | 34 | |
| Lucas Giolito | SP | Bos | 30 | |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Grant Holmes | SP | Atl | N/A | |
| Dustin May | SP | LAD | 27 | |
| Reese Olson | SP | Det | 25 | |
| Cole Ragans | SP | KC | 27 | |
| Shane Smith | SP | ChA | N/A | |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Justin Martinez | RP | Ari | 23 | May 01, 2025:15 Day IL |
| Andres Munoz | RP | Sea | 26 | |
| Cade Smith | RP | Cle | N/A |