| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Agustin Ramirez | C | Mia | 23 | |
| Austin Wells | C | NYA | 25 | |
| Pete Alonso | 1B | NYN | 30 | |
| Vladimir Guerrero Jr. | 1B | Tor | 26 | |
| Nick Kurtz | 1B | Oak | 22 | |
| Kyle Manzardo | 1B | Cle | 24 | |
| Luisangel Acuna | 2B | NYN | 23 | |
| Kristian Campbell | 2B (OF) | Bos | 22 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Francisco Lindor | SS | NYN | 31 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Caleb Durbin | 3B | Mil | 25 | |
| Cam Smith | 3B (OF) | Hou | 22 | |
| Jordan Westburg | 3B (2B) | Bal | 26 | April 28, 2025:10 Day IL |
| Yordan Alvarez | OF | Hou | 27 | |
| Michael Conforto | OF | LAD | 32 | |
| Pete Crow-Armstrong | OF | ChN | 23 | |
| Tommy Edman | OF (2B) | LAD | 29 | |
| Fernando Tatis Jr. | OF | SD | 26 | |
| Walker Buehler | SP | Bos | 30 | May 02, 2025:15 Day IL-Shoulder |
| Garrett Crochet | SP | Bos | 25 | |
| Luis Gil | SP | NYA | 26 | March 24, 2025:60 Day IL |
| Clay Holmes | SP | NYN | 32 | |
| Cade Horton | SP | ChN | 23 | |
| Jackson Jobe | SP | Det | 22 | |
| Jack Leiter | SP | Tex | N/A | |
| Jesus Luzardo | SP | Phi | 27 | |
| Landen Roupp | SP | SF | N/A | |
| David Bednar | RP | Pit | 30 | |
| Aroldis Chapman | RP | Bos | 37 | |
| Emmanuel Clase | RP | Cle | 27 | |
| Luke Jackson | RP | Tex | 33 | |
| Tommy Kahnle | RP | Det | 35 |