| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Keibert Ruiz | C | Was | 26 | |
| Dalton Rushing | C | LAD | 24 | |
| Pete Alonso | 1B | NYN | 30 | |
| Deyvison De Los Santos | 1B | Mia | 21 | |
| Colt Keith | 1B (2B) | Det | 23 | |
| Matt Mervis | 1B | Mia | 27 | |
| Luisangel Acuna | 2B | NYN | 23 | |
| Ozzie Albies | 2B | Atl | 28 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Javier Sanoja | SS (OF) | Mia | N/A | |
| Ezequiel Tovar | SS | Col | 23 | April 19, 2025:10 Day IL |
| Junior Caminero | 3B | TB | 21 | |
| Austin Riley | 3B | Atl | 28 | |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Jarren Duran | OF | Bos | 28 | |
| Jackson Merrill | OF | SD | 22 | April 08, 2025:10 Day IL |
| Kameron Misner | OF | TB | N/A | |
| Brent Rooker | OF | Oak | 30 | |
| Juan Soto | OF | NYN | 26 | |
| Alex Verdugo | OF | Atl | 28 | |
| Matt Wallner | OF | Min | 27 | April 17, 2025:10 Day IL |
| Sandy Alcantara | SP | Mia | 29 | |
| Hunter Dobbins | SP | Bos | N/A | |
| Richard Fitts | SP | Bos | N/A | April 13, 2025:15 Day IL |
| Clay Holmes | SP | NYN | 32 | |
| Michael King | SP | SD | 29 | |
| Pablo Lopez | SP | Min | 29 | |
| Bailey Ober | SP | Min | 29 | |
| AJ Smith-Shawver | SP | Atl | 22 | |
| Spencer Strider | SP | Atl | 26 | April 21, 2025:15 Day IL |
| Luke Jackson | RP | Tex | 33 | |
| Justin Martinez | RP | Ari | 23 | May 01, 2025:15 Day IL |
| Andres Munoz | RP | Sea | 26 | |
| Tanner Scott | RP | LAD | 30 |