| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Samuel Basallo | C | Bal | 20 | |
| Jonah Heim | C | Tex | 29 | |
| Shea Langeliers | C | Oak | 27 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Bryce Harper | 1B | Phi | 32 | |
| Michael Toglia | 1B | Col | 26 | |
| Andrés Gimenez | 2B | Tor | 26 | |
| Romy Gonzalez | 2B (1B) | Bos | 28 | |
| Brandon Lowe | 2B | TB | 30 | |
| Jeff McNeil | 2B | NYN | 33 | |
| J.P. Crawford | SS | Sea | 30 | |
| Francisco Lindor | SS | NYN | 31 | |
| Nolan Arenado | 3B | StL | 34 | |
| Caleb Durbin | 3B | Mil | 25 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Roman Anthony | OF | Bos | 20 | |
| Randy Arozarena | OF | Sea | 30 | |
| Jordan Beck | OF | Col | 24 | |
| Cody Bellinger | OF | NYA | 29 | |
| Pete Crow-Armstrong | OF | ChN | 23 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Taylor Ward | OF | LAA | 31 | |
| Bubba Chandler | SP | Pit | N/A | |
| Jacob deGrom | SP | Tex | 36 | |
| Max Fried | SP | NYA | 31 | |
| Zac Gallen | SP | Ari | 29 | |
| MacKenzie Gore | SP | Was | 26 | |
| Shane McClanahan | SP | TB | 28 | March 26, 2025:60 Day IL-Ab strain |
| Eury Perez | SP | Mia | 22 | February 20, 2025:60 Day IL |
| Joe Ryan | SP | Min | 28 | |
| Cristopher Sanchez | SP | Phi | 28 | |
| Yoshinobu Yamamoto | SP | LAD | 26 | |
| Felix Bautista | RP | Bal | 29 | |
| Trevor Megill | RP | Mil | 31 |