| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| William Contreras | C | Mil | 27 | |
| Ryan Jeffers | C | Min | 27 | |
| Michael Busch | 1B | ChN | 27 | |
| Matt Olson | 1B | Atl | 31 | |
| Eric Wagaman | 1B (OF) | Mia | N/A | |
| Brendan Donovan | 2B (SS,OF) | StL | 28 | |
| Thairo Estrada | 2B | Col | 29 | March 27, 2025:60 Day IL |
| Nico Hoerner | 2B | ChN | 27 | |
| Zach McKinstry | 2B (3B,OF) | Det | 30 | |
| Geraldo Perdomo | SS | Ari | 25 | |
| Bobby Witt Jr. | SS | KC | 24 | |
| Jace Jung | 3B | Det | 24 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Matt Vierling | 3B | Det | 28 | March 27, 2025:10 Day IL |
| Max Clark | OF | Det | 20 | |
| Adolis Garcia | OF | Tex | 32 | |
| Michael Harris II | OF | Atl | 24 | |
| Christopher Morel | OF | TB | 25 | |
| Brandon Nimmo | OF | NYN | 32 | |
| Luis Robert Jr. | OF | ChA | 27 | |
| Jose Berrios | SP | Tor | 30 | |
| Taj Bradley | SP | TB | 24 | |
| Luis Castillo | SP | Sea | 32 | |
| Jack Flaherty | SP | Det | 29 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Jack Leiter | SP | Tex | N/A | |
| Pablo Lopez | SP | Min | 29 | |
| Tyler Mahle | SP | Tex | 30 | |
| Paul Skenes | SP | Pit | 22 | |
| Spencer Strider | SP | Atl | 26 | April 21, 2025:15 Day IL |
| Simeon Woods Richardson | SP | Min | 24 | |
| Felix Bautista | RP | Bal | 29 | |
| David Bednar | RP | Pit | 30 | |
| Tommy Kahnle | RP | Det | 35 |