| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Cal Raleigh | C | Sea | 28 | |
| Jonathan Aranda | 1B | TB | 26 | |
| Jac Caglianone | 1B | KC | 22 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Matt Olson | 1B | Atl | 31 | |
| Ben Rice | 1B | NYA | 26 | |
| Travis Bazzana | 2B | Cle | 22 | |
| Nick Gonzales | 2B | Pit | 25 | March 28, 2025:10 Day IL |
| Christian Moore | 2B | LAA | 22 | |
| Gabriel Arias | SS (2B,3B) | Cle | 25 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Dansby Swanson | SS | ChN | 31 | |
| Coby Mayo | 3B | Bal | 23 | |
| Ryan McMahon | 3B | Col | 30 | |
| Yoan Moncada | 3B | LAA | 29 | April 10, 2025:10 Day IL |
| Andrew Benintendi | OF | ChA | 30 | |
| Lawrence Butler | OF | Oak | 24 | |
| Jake Fraley | OF | Cin | 29 | |
| Hunter Goodman | OF (C) | Col | 25 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Matt Wallner | OF | Min | 27 | April 17, 2025:10 Day IL |
| James Wood | OF | Was | 22 | |
| Kris Bubic | SP | KC | 27 | |
| MacKenzie Gore | SP | Was | 26 | |
| Grant Holmes | SP | Atl | N/A | |
| Dustin May | SP | LAD | 27 | |
| Reese Olson | SP | Det | 25 | |
| Luis Ortiz | SP | Cle | 26 | |
| Cole Ragans | SP | KC | 27 | |
| Shane Smith | SP | ChA | N/A | |
| Yoshinobu Yamamoto | SP | LAD | 26 | |
| Jeff Hoffman | RP | Tor | 32 | |
| Mason Miller | RP | Oak | 26 | |
| Andres Munoz | RP | Sea | 26 |