| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Samuel Basallo | C | Bal | 20 | |
| Edgar Quero | C | ChA | 22 | |
| Agustin Ramirez | C | Mia | 23 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Ryan Mountcastle | 1B | Bal | 28 | |
| Nolan Schanuel | 1B | LAA | 23 | |
| Christian Walker | 1B | Hou | 34 | |
| Jazz Chisholm Jr. | 2B | NYA | 27 | May 02, 2025:15 Day IL-Oblique |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Gavin Lux | 2B (OF) | Cin | 27 | |
| Max Muncy | SS (2B) | Oak | 22 | |
| Corey Seager | SS | Tex | 31 | April 23, 2025:10 Day IL |
| Anthony Volpe | SS | NYA | 24 | |
| Nolan Arenado | 3B | StL | 34 | |
| Alec Bohm | 3B | Phi | 28 | |
| Wilyer Abreu | OF | Bos | 25 | |
| Byron Buxton | OF | Min | 31 | |
| Evan Carter | OF | Tex | 22 | |
| Sal Frelick | OF | Mil | 25 | |
| Trent Grisham | OF | NYA | 28 | |
| Victor Scott | OF | StL | 24 | |
| Jorge Soler | OF | LAA | 32 | |
| Colby Thomas | OF | Oak | 24 | |
| Daulton Varsho | OF | Tor | 28 | |
| Erick Fedde | SP | StL | 32 | |
| Nick Lodolo | SP | Cin | 27 | |
| Zebby Matthews | SP | Min | N/A | |
| Tylor Megill | SP | NYN | 29 | |
| Bryce Miller | SP | Sea | 26 | |
| Robbie Ray | SP | SF | 33 | |
| José Soriano | SP | LAA | 26 | |
| Spencer Strider | SP | Atl | 26 | April 21, 2025:15 Day IL |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Raisel Iglesias | RP | Atl | 35 |